Regulation of blood-testis barrier assembly in vivo by germ cells.

ABSTRACT: The assembly of the blood-testis barrier (BTB) during postnatal development is crucial to support meiosis. However, the role of germ cells in BTB assembly remains unclear. Herein, Kit^W/Kit^WV mice were used as a study model. These mice were infertile, failing to establish a functional BTB to support meiosis due to c-Kit mutation. Transplantation of undifferentiated spermatogonia derived from normal mice into the testis of Kit^W/Kit^WV mice triggered functional BTB assembly, displaying cyclic remodeling during the epithelial cycle. Also, transplanted germ cells were capable of inducing Leydig cell testosterone production, which could enhance the expression of integral membrane protein claudin 3 in Sertoli cells. Early spermatocytes were shown to play

SUBMITTER: Li XY 

PROVIDER: S-EPMC6137450 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature