Impact of nanoparticle surface functionalization on the protein corona and cellular adhesion, uptake and transport.

ABSTRACT:

Background

Upon ingestion, nanoparticles can interact with the intestinal epithelial barrier potentially resulting in systemic uptake of nanoparticles. Nanoparticle properties have been described to influence the protein corona formation and subsequent cellular adhesion, uptake and transport. Here, we aimed to study the effects of nanoparticle size and surface chemistry on the protein corona formation and subsequent cellular adhesion, uptake and transport. Caco-2 intestinal cells, were exposed to negatively charged polystyrene nanoparticles (PSNPs) (50 and 200 nm), functionalized with sulfone or carboxyl groups, at nine nominal concentrations (15-250 μg/ml) for 10 up to 120 min. The protein coronas were analysed by LC-MS/MS.

Results

Subtle differences in the protein composit

SUBMITTER: Abdelkhaliq A 

PROVIDER: S-EPMC6138932 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature