Small RNA and degradome sequencing used to elucidate the basis of tolerance to salinity and alkalinity in wheat.

ABSTRACT:

Background

Soil salinity and/or alkalinity impose a major constraint over crop yield and quality. An understanding of the molecular basis of the plant response to these stresses could inform the breeding of more tolerant varieties. The bread wheat cultivar SR3 exhibits an enhanced level of salinity tolerance, while SR4 is distinguished by its superior tolerance of alkalinity.

Results

The small RNA and degradome sequencing was used to explore the miRNAs and corresponding targets associated with the superior stress tolerance of the SR lines. An examination of the small RNA content of these two closely related lines revealed the presence of 98 known and 219 novel miRNA sequences. Degradome libraries were constructed in order to identify the targets of the miRNAs, leading to the

SUBMITTER: Han H 

PROVIDER: S-EPMC6139162 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature