Mast cells and mast cell tryptase enhance migration of human lung fibroblasts through protease-activated receptor 2.

ABSTRACT:

Background

Mast cells may activate fibroblasts and contribute to remodeling processes in the lung. However, the mechanism behind these actions needs to be further investigated. Fibroblasts are major regulators of on-going remodeling processes. Protease activated receptor 2 (PAR2) expressed by fibroblasts may be activated by serine proteases, such as the mast cell mediator tryptase. The objective in this study was to investigate the effects of mast cells and specifically mast cell tryptase on fibroblast migration and the role of PAR2 activation.

Methods

Human lung fibroblasts (HFL-1) were cultured together with human peripheral blood-derived mast cells or LAD2 mast cells and stimulated with either conditioned medium from LAD2 cells or tryptase. Analyses of immunological stimu

SUBMITTER: Bagher M 

PROVIDER: S-EPMC6139170 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature