A Requirement for Global Transcription Factor Lrp in Licensing Replication of Vibrio cholerae Chromosome 2.

ABSTRACT: The human pathogen, Vibrio cholerae, belongs to the 10% of bacteria in which the genome is divided. Each of its two chromosomes, like bacterial chromosomes in general, replicates from a unique origin at fixed times in the cell cycle. Chr1 initiates first, and upon duplication of a site in Chr1, crtS, Chr2 replication initiates. Recent in vivo experiments demonstrate that crtS binds the Chr2-specific initiator RctB and promotes its initiator activity by remodeling it. Compared to the well-defined RctB binding sites in the Chr2 origin, crtS is an order of magnitude longer, suggesting that other factors can bind to it. We developed an in vivo screen to identify additional crtS-binding proteins and identified the global transcription factor, Lrp

SUBMITTER: Ciaccia PN 

PROVIDER: S-EPMC6139311 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature