Folding and Lipid Composition Determine Membrane Interaction of the Disordered Protein COR15A.

ABSTRACT: Plants from temperate climates, such as the model plant Arabidopsis thaliana, are challenged with seasonal low temperatures that lead to increased freezing tolerance in fall in a process termed cold acclimation. Among other adaptations, this involves the accumulation of cold-regulated (COR) proteins, such as the intrinsically disordered chloroplast-localized protein COR15A. Together with its close homolog COR15B, it stabilizes chloroplast membranes during freezing. COR15A folds into amphipathic α-helices in the presence of high concentrations of low-molecular-mass crowders or upon dehydration. Under these conditions, the (partially) folded protein binds peripherally to membranes. In our study, we have used coarse-grained molecular dynamics simulations to elucidate the details of COR15A-mem

SUBMITTER: Navarro-Retamal C 

PROVIDER: S-EPMC6139882 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature