Nano LC-MS using capillary columns enables accurate quantification of modified ribonucleosides at low femtomol levels.

ABSTRACT: Post-transcriptional chemical modifications of (t)RNA molecules are crucial in fundamental biological processes, such as translation. Despite their biological importance and accumulating evidence linking them to various human diseases, technical challenges have limited their detection and accurate quantification. Here, we present a sensitive capillary nanoflow liquid chromatography mass spectrometry (nLC-MS) pipeline for quantitative high-resolution analysis of ribonucleoside modifications from complex biological samples. We evaluated two porous graphitic carbon (PGC) materials and one end-capped C18 reference material as stationary phases for reversed-phase separation. We found that these matrices have complementing retention and separation characteristics, including the capability to sep

SUBMITTER: Sarin LP 

PROVIDER: S-EPMC6140458 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature