Queuosine modification protects cognate tRNAs against ribonuclease cleavage.

ABSTRACT: Eukaryotic transfer RNAs (tRNA) contain on average 13 modifications that perform a wide range of roles in translation and in the generation of tRNA fragments that regulate gene expression. Queuosine (Q) modification occurs in the wobble anticodon position of tRNAs for amino acids His, Asn, Tyr, and Asp. In eukaryotes, Q modification is fully dependent on diet or on gut microbiome in multicellular organisms. Despite decades of study, cellular roles of Q modification remain to be fully elucidated. Here we show that in human cells, Q modification specifically protects its cognate tRNA^His and tRNA^Asn against cleavage by ribonucleases. We generated cell lines that contain completely depleted or fully Q-modified tRNAs. Using these resources, we found that Q modification sig

SUBMITTER: Wang X 

PROVIDER: S-EPMC6140461 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature