Increasing the length of poly-pyrimidine bulges broadens RNA conformational ensembles with minimal impact on stacking energetics.

ABSTRACT: Helical elements separated by bulges frequently undergo transitions between unstacked and coaxially stacked conformations during the folding and function of noncoding RNAs. Here, we examine the dynamic properties of poly-pyrimidine bulges of varying length (n = 1-4, 7) across a range of Mg²⁺ concentrations using HIV-1 TAR RNA as a model system and solution NMR spectroscopy. In the absence of Mg²⁺, helices linked by bulges with n ≥ 3 residues adopt predominantly unstacked conformations (stacked population <15%), whereas one-bulge and two-bulge motifs adopt predominantly stacked conformations (stacked population >74%). In the presence of 3 mM Mg²⁺, the helices predominantly coaxially stack (stacked population >84%), regardless of bulge length, a

SUBMITTER: Merriman DK 

PROVIDER: S-EPMC6140463 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature