Contribution of Fluorescence Techniques in Determining the Efficiency of the Non-thermal Plasma Treatment.

ABSTRACT: We have recently developed a non-thermal plasma (NTP) equipment intended to sterilize fragile medical devices and maintain the sterile state of items downstream the treatment. With traditional counts on agar plate a six log reduction of Staphylococcus aureus viability was obtained within 120 min of O₂, Ar, or N₂ NTP treatments. However to determine the best NTP process, we studied the different physiological states of S. aureus by flow cytometry (FC) and confocal laser scanning microscopy (CLSM) focusing on the esterasic activity and membrane integrity of the bacteria. Two fluorochromes, 5-(and-6)-carboxy-2^',7^'-dichlorofluorescein diacetate and propidium iodide were used in order to distinguish three sub-populations: metabolically a

SUBMITTER: Carre G 

PROVIDER: S-EPMC6140754 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature