Cultured equine satellite cells as a model system to assess leucine stimulated protein synthesis in horse muscle.

ABSTRACT: Leucine has been shown to stimulate the mammalian/mechanistic target of rapamycin (mTOR) signaling pathway which plays numerous key regulatory roles in cell growth, survival, and metabolism including protein synthesis in a number of species. However, previous work with equine satellite cells has suggested distinct species differences in regards to physiological effects and the magnitude of responses to growth factors and regulators. Because there is limited research available regarding the role of leucine in regulating equine skeletal muscle protein synthesis, the objective of this study was to evaluate the effect of leucine on the mTOR signaling pathway in cultured equine satellite. Protein synthesis was evaluated by measuring the incorporation of [3H] Phenylalanine (3HPhe) in equine sate

SUBMITTER: DeBoer ML 

PROVIDER: S-EPMC6140856 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature