A mouse model engineered to conditionally express the progesterone receptor-B isoform.

ABSTRACT: Using a Rosa26 gene targeting strategy in mouse embryonic stem cells, we have generated a new transgenic mouse (Pgr-B ^LSL ), which is designed to conditionally express the epitope-tagged mouse progesterone receptor-B (PGR-B) isoform when crossed with a specific cre driver mouse. To functionally validate this transgenic mouse, we crossed the Pgr-B ^LSL mouse with the MMTV-CREA transgenic mouse to create the MMTV-CREA/Pgr-B ^LSL bigenic (termed PR-B:OE to denote PGR-B overexpressor). As expected, transgene-derived PGR-B protein was specifically targeted to the virgin mammary gland epithelium. At a functional level, the PR-B:OE bigenic exhibited abnormal mammary morphogenesis-dilated epithelial ducts, precocious alveologenesis and lateral side-branching, along w

SUBMITTER: Hai L 

PROVIDER: S-EPMC6141023 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature