Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia.

ABSTRACT:

Aim

This study aimed to analyze the amino acid sequence of Cytochrome b (Cyt b) gene in Kejobong goat and its genetic relationships with local goats located in Asia.

Materials and methods

A total of 28 heads of Kejobong goat were purposively sampled. The deoxyribonucleic acid (DNA) was extracted from blood using gSYNC DNA mini kit (Geneaid Biotech Ltd.). Cyt b gene was amplified using polymerase chain reaction (PCR) method with CytbCapF and CytbCapR primers. The amplified PCR products were sequenced for further analysis.

Results

There were a total 377 amino acid sequences translated from 1140 base pair (bp) of Cyt b gene, 99.20% of it were monomorphic, amino acid alterations were found at site 16^th, 121^st, and 231

SUBMITTER: Lestari DA 

PROVIDER: S-EPMC6141281 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature