Dataset Information

Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia.

ABSTRACT:

Aim

This study aimed to analyze the amino acid sequence of Cytochrome b (Cyt b) gene in Kejobong goat and its genetic relationships with local goats located in Asia.

Materials and methods

A total of 28 heads of Kejobong goat were purposively sampled. The deoxyribonucleic acid (DNA) was extracted from blood using gSYNC DNA mini kit (Geneaid Biotech Ltd.). Cyt b gene was amplified using polymerase chain reaction (PCR) method with CytbCapF and CytbCapR primers. The amplified PCR products were sequenced for further analysis.

Results

There were a total 377 amino acid sequences translated from 1140 base pair (bp) of Cyt b gene, 99.20% of it were monomorphic, amino acid alterations were found at site 16^th, 121^st, and 231^st, and Kejobong goat was in the same cluster with Southeast Asian local goats.

Conclusion

Most of the amino acid sequence on Cyt b gene in Kejobong goat is monomorphic (99.20%), only a few nucleotide mutations were found that causing amino acid alteration in three sites (0.80%). Kejobong goat has a close genetic relationship to several local goats in Southeast Asian.

SUBMITTER: Lestari DA

PROVIDER: S-EPMC6141281 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Publications

Amino acid sequence based on <i>Cytochrome b</i> gene in Kejobong goat and its genetic relationships among several local goats in Asia.

Lestari Dela Ayu DA Purbowati Endang E Sutopo Sutopo S Kurnianto Edy E

Veterinary world 20180830 8

<h4>Aim</h4>This study aimed to analyze the amino acid sequence of <i>Cytochrome b</i> (<i>Cyt b</i>) gene in Kejobong goat and its genetic relationships with local goats located in Asia.<h4>Materials and methods</h4>A total of 28 heads of Kejobong goat were purposively sampled. The deoxyribonucleic acid (DNA) was extracted from blood using gSYNC DNA mini kit (Geneaid Biotech Ltd.). <i>Cyt b</i> gene was amplified using polymerase chain reaction (PCR) method with CytbCapF and CytbCapR primers. T ...[more]

PMID: 30250384

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