An epidemiological investigation on occurrence of enterohemorrhagic Escherichia coli in raw milk.

ABSTRACT:

Aim

The aim of the present investigation was to study the epidemiology of enterohemorrhagic Escherichia coli (EHEC) in raw milk and molecular characterization of isolates using multiplex polymerase chain reaction (PCR).

Materials and methods

A total of 125 raw milk samples were subjected to isolation, identification, and confirmation of virulence-associated genes by multiplex PCR (mPCR). The samples were collected from a milk cooperative society of Thrissur district, Kerala. For further epidemiological investigation, samples such as dung (126), hair coat of cow (60), udder swab (60), udder wash (60), milking utensil wash (36), Milker's hand wash (36), water (36), soil (36), and feed (36) were collected from the households from which the raw milk tested positive for EH

SUBMITTER: Vanitha HD 

PROVIDER: S-EPMC6141287 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature