Directed evolution of excited state lifetime and brightness in FusionRed using a microfluidic sorter.

ABSTRACT: Green fluorescent proteins (GFP) and their blue, cyan and red counterparts offer unprecedented advantages as biological markers owing to their genetic encodability and straightforward expression in different organisms. Although significant advancements have been made towards engineering the key photo-physical properties of red fluorescent proteins (RFPs), they continue to perform sub-optimally relative to GFP variants. Advanced engineering strategies are needed for further evolution of RFPs in the pursuit of improving their photo-physics. In this report, a microfluidic sorter that discriminates members of a cell-based library based on their excited state lifetime and fluorescence intensity is used for the directed evolution of the photo-physical properties of FusionRed. In-flow measurement

SUBMITTER: Manna P 

PROVIDER: S-EPMC6141309 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature