Antibiotic Effects on Microbial Communities Responsible for Denitrification and N₂O Production in Grassland Soils.

ABSTRACT: Antibiotics in soils may affect the structure and function of microbial communities. In this study, we investigated the acute effects of tetracycline on soil microbial community composition and production of nitrous oxide (N₂O) and dinitrogen (N₂) as the end-products of denitrification. Grassland soils were pre-incubated with and without tetracycline for 1-week prior to measurements of N₂O and N₂ production in soil slurries along with the analysis of prokaryotic and fungal communities by quantitative polymerase chain reaction (qPCR) and next-generation sequencing. Abundance and taxonomic composition of bacteria carrying two genotypes of N₂O reductase genes (nosZ-I and nosZ-II) were evaluated through qPCR and metabolic inf

SUBMITTER: Semedo M 

PROVIDER: S-EPMC6141661 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature