Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus.

ABSTRACT: Foot-and-mouth disease virus (FMDV) has a wide host range. Its pathogenesis varies among hosts and types of viruses. Most investigations of pathogenesis have been performed on cattle and swine. However, FMDV research in cattle is hampered by the lack of a stable in vitro infection model. In this study, the stable bovine thyroid (BTY) cell line hTERT-BTY from primary BTY cells was established by telomerase reverse transcriptase over expression. The results of karyotype analysis and experiments on morphological and biological characteristics indicated that this cell line possessed the qualities of primary BTY cells, which could be extended indefinitely with stable morphology and steady growth rates. The hTERT-BTY cell line, has 60 chromosomes including 29 pairs of autosomes and 1 pair

SUBMITTER: Mao R 

PROVIDER: S-EPMC6141800 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature