Three-dimensional virtual histology of human cerebellum by X-ray phase-contrast tomography.

ABSTRACT: To quantitatively evaluate brain tissue and its corresponding function, knowledge of the 3D cellular distribution is essential. The gold standard to obtain this information is histology, a destructive and labor-intensive technique where the specimen is sliced and examined under a light microscope, providing 3D information at nonisotropic resolution. To overcome the limitations of conventional histology, we use phase-contrast X-ray tomography with optimized optics, reconstruction, and image analysis, both at a dedicated synchrotron radiation endstation, which we have equipped with X-ray waveguide optics for coherence and wavefront filtering, and at a compact laboratory source. As a proof-of-concept demonstration we probe the 3D cytoarchitecture in millimeter-sized punches of unstained human

SUBMITTER: Topperwien M 

PROVIDER: S-EPMC6142271 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature