Regulation of ATP levels in Escherichia coli using CRISPR interference for enhanced pinocembrin production.

ABSTRACT:

Background

Microbial biosynthesis of natural products holds promise for preclinical studies and treating diseases. For instance, pinocembrin is a natural flavonoid with important pharmacologic characteristics and is widely used in preclinical studies. However, high yield of natural products production is often limited by the intracellular cofactor level, including adenosine triphosphate (ATP). To address this challenge, tailored modification of ATP concentration in Escherichia coli was applied in efficient pinocembrin production.

Results

In the present study, a clustered regularly interspaced short palindromic repeats (CRISPR) interference system was performed for screening several ATP-related candidate genes, where metK and proB showed its potential to improve ATP level and

SUBMITTER: Tao S 

PROVIDER: S-EPMC6142380 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature