Novel antibodies against GPIbα inhibit pulmonary metastasis by affecting vWF-GPIbα interaction.

ABSTRACT:

Background

Platelet glycoprotein Ibα (GPIbα) extracellular domain, which is part of the receptor complex GPIb-IX-V, plays an important role in tumor metastasis. However, the mechanism through which GPIbα participates in the metastatic process remains unclear. In addition, potential bleeding complication remains an obstacle for the clinical use of anti-platelet agents in cancer therapy.

Methods

We established a series of screening models and obtained rat anti-mouse GPIbα monoclonal antibodies (mAb) 1D12 and 2B4 that demonstrated potential value in suppressing cancer metastasis. To validate our findings, we further obtained mouse anti-human GPIbα monoclonal antibody YQ3 through the same approach.

Results

1D12 and 2B4 affected the von Willebrand factor (vWF)-GPIbα intera

SUBMITTER: Qi Y 

PROVIDER: S-EPMC6142402 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature