Improved systemic metabolism and adipocyte biology in miR-150 knockout mice.

ABSTRACT:

Introduction

Short non-coding micro-RNAs (miRNAs) are post-transcriptional factors that directly regulate protein expression by degrading or inhibiting target mRNAs; however, the role of miRNAs in obesity and cardiometabolic disease remains unclarified. Based on our earlier study demonstrating that miR-150 influences lipid metabolism, we have studied effects of miR-150 on systemic metabolism and adipocyte biology.

Materials and methods

Metabolic phenotypes including body weight, food intake, body composition, glucose tolerance and insulin sensitivity were assessed in WT and global miR-150 KO male mice fed a high-fat diet. Molecular changes in epididymal adipose tissue were evaluated through qRT-PCR and Western blotting.

Results

miR-150 KO mice displayed lower body wei

SUBMITTER: Kang M 

PROVIDER: S-EPMC6142816 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature