Six domesticated PiggyBac transposases together carry out programmed DNA elimination in <i>Paramecium</i>.

ABSTRACT: The domestication of transposable elements has repeatedly occurred during evolution and domesticated transposases have often been implicated in programmed genome rearrangements, as remarkably illustrated in ciliates. In Paramecium, PiggyMac (Pgm), a domesticated PiggyBac transposase, carries out developmentally programmed DNA elimination, including the precise excision of tens of thousands of gene-interrupting germline Internal Eliminated Sequences (IESs). Here, we report the discovery of five groups of distant Pgm-like proteins (PgmLs), all able to interact with Pgm and essential for its nuclear localization and IES excision genome-wide. Unlike Pgm, PgmLs lack a conserved catalytic site, suggesting that they rather have an architectural function within a multi-component excision co

SUBMITTER: Bischerour J 

PROVIDER: S-EPMC6143343 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature