Mycofactocin Biosynthesis Proceeds through 3-Amino-5-[( p-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone (AHDP); Direct Observation of MftE Specificity toward MftA.

ABSTRACT: The structure of the ribosomally synthesized and post-translationally modified peptide product mycofactocin is unknown. Recently, the first step in mycofactocin biosynthesis was shown to be catalyzed by MftC in two S-adenosylmethionine-dependent steps. In the first step, MftC catalyzes the oxidative decarboxylation of the MftA peptide to produce the styrene-containing intermediate MftA**, followed by a subsequent C-C bond formation to yield the lactam-containing MftA*. Here, we demonstrate the subsequent biosynthetic step catalyzed by MftE is specific for MftA*. The hydrolysis of MftA* leads to the formation of MftA(1-28) and 3-amino-5-[( p-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone (AHDP). The hydrolysis reaction is Fe²⁺-dependent, and addition of the metal to the reaction mixture leads to a k_obs of ∼0.2 min^-1. Lastly, we validate the structure of AHDP by ¹H, ¹³C, and COSY nuclear magnetic resonance techniques as well as mass spectrometry.

SUBMITTER: Ayikpoe R 

PROVIDER: S-EPMC6143433 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature