Aqueous mounting media increasing tissue translucence improve image quality in Structured Illumination Microscopy of thick biological specimen.

ABSTRACT: Structured Illumination Microscopy (SIM) is a super-resolution microscopy method that has significantly advanced studies of cellular structures. It relies on projection of illumination patterns onto a fluorescently labelled biological sample. The information derived from the sample is then shifted to a detectable band, and in the process of image calculation in Fourier space the resolution is doubled. Refractive index homogeneity along the optical path is crucial to maintain a highly modulated illumination pattern necessary for high-quality SIM. This applies in particular to thick samples consisting of large cells and tissues. Surprisingly, sample mounting media for SIM have not undergone a significant evolution for almost a decade. Through identification and systematic evaluation of a num

SUBMITTER: Szczurek A 

PROVIDER: S-EPMC6143540 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature