Circulating monocytes from prostate cancer patients promote invasion and motility of epithelial cells.

ABSTRACT:

Background

Recruited myeloid cells are known to promote cancer initiation, malignant progression, metastasis, and resistance to therapy in the tumor niche. We tested the hypothesis that circulating blood monocytes from advanced prostate cancer (PCa) patients exhibit a protumor phenotype and directly influence the tumor microenvironment in response to tumor-derived signals.

Methods

Blood monocytes from advanced and stable PCa patients were cultured, and the conditioned media (CM) were collected and analyzed using standard invasion and wound closure assays to measure effects on invasion and motility of PCa tumor cells. We then identified the proteome profile of these monocytes using proteome array and ELISA.

Results

Conditioned media from circulating monocytes in patien

SUBMITTER: Cavassani KA 

PROVIDER: S-EPMC6143932 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature