MiR-335-5p restores cisplatin sensitivity in ovarian cancer cells through targeting BCL2L2.

ABSTRACT:

Objective

Our study was designed to explore the association miR-335-5p and BCL2L2 and to investigate the influence of miR-335-5p/BCL2L2 axis on cisplatin-resistant ovarian cancer cells.

Methods

Microarray analysis was used to determine differentially expressed microRNAs in primary and cisplatin-resistant A2780 cells. Cell function experiments were conducted to investigate the effect of miR-335-5p on the cisplatin sensitivity of A2780 cells. The targeted relationship between BCL2L2 mRNA and miR-335-5p was validated through luciferase assay. Tumor xenograft was performed to confirm the function of miR-335-5p in restoring the cisplatin sensitivity of the ovarian cancer cells.

Results

MiR-335-5p was lowly expressed in cisplatin-resistant A2780 cells. Overexpression of miR

SUBMITTER: Liu R 

PROVIDER: S-EPMC6143943 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature