DNA binding with a minimal scaffold: structure-function analysis of Lig E DNA ligases.

ABSTRACT: DNA ligases join breaks in the phosphodiester backbone of DNA by catalysing the formation of bonds between opposing 5'P and 3'OH ends in an adenylation-dependent manner. Catalysis is accompanied by reorientation of two core domains to provide access to the active site for cofactor utilization and enable substrate binding and product release. The general paradigm is that DNA ligases engage their DNA substrate through complete encirclement of the duplex, completed by inter-domain kissing contacts via loops or additional domains. The recent structure of a minimal Lig E-type DNA ligase, however, implies it must use a different mechanism, as it lacks any domains or loops appending the catalytic core which could complete encirclement. In the present study, we have used a structure-guided mutagen

SUBMITTER: Williamson A 

PROVIDER: S-EPMC6144786 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature