A novel λ integrase-mediated seamless vector transgenesis platform for therapeutic protein expression.

ABSTRACT: Advances in stem cell engineering, gene therapy and molecular medicine often involve genome engineering at a cellular level. However, functionally large or multi transgene cassette insertion into the human genome still remains a challenge. Current practices such as random transgene integration or targeted endonuclease-based genome editing are suboptimal and might pose safety concerns. Taking this into consideration, we previously developed a transgenesis tool derived from phage λ integrase (Int) that precisely recombines large plasmid DNA into an endogenous sequence found in human Long INterspersed Elements-1 (LINE-1). Despite this advancement, biosafety concerns associated with bacterial components of plasmids, enhanced uptake and efficient transgene expression remained problematic. We th

SUBMITTER: Makhija H 

PROVIDER: S-EPMC6144826 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature