EDNA metabarcoding as a new surveillance approach for coastal Arctic biodiversity.

ABSTRACT: Because significant global changes are currently underway in the Arctic, creating a large-scale standardized database for Arctic marine biodiversity is particularly pressing. This study evaluates the potential of aquatic environmental DNA (eDNA) metabarcoding to detect Arctic coastal biodiversity changes and characterizes the local spatio-temporal distribution of eDNA in two locations. We extracted and amplified eDNA using two COI primer pairs from ~80 water samples that were collected across two Canadian Arctic ports, Churchill and Iqaluit, based on optimized sampling and preservation methods for remote regions surveys. Results demonstrate that aquatic eDNA surveys have the potential to document large-scale Arctic biodiversity change by providing a rapid overview of coastal metazoan biodi

SUBMITTER: Lacoursiere-Roussel A 

PROVIDER: S-EPMC6144963 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature