Changes in miRNA Gene Expression during Wound Repair in Differentiated Normal Human Bronchial Epithelium.

ABSTRACT:

Purpose

Airway epithelium acts as a protective barrier against the particles from the inhaled air. Damage to the epithelium may result in loss of the barrier function. Epithelial repair in response to injury requires complex mechanisms, such as microRNA, small noncoding molecules, to regulate the processes involved in wound repair. We aimed to establish if the microRNA gene expression profile is altered during the airway epithelial repair in differentiated cells.

Methods

miRNA gene expression profile during the wound closure of differentiated normal human bronchial epithelium (NHBE) from one donor was analysed using quantitative real-time PCR. We have analysed the expression of 754 genes at five time points during a 48-hour period of epithelium repair using TaqMan Low Densit

SUBMITTER: Narozna B 

PROVIDER: S-EPMC6145058 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature