Kinetics of mRNA delivery and protein translation in dendritic cells using lipid-coated PLGA nanoparticles.

ABSTRACT:

Background

Messenger RNA (mRNA) has gained remarkable attention as an alternative to DNA-based therapies in biomedical research. A variety of biodegradable nanoparticles (NPs) has been developed including lipid-based and polymer-based systems for mRNA delivery. However, both systems still lack in achieving an efficient transfection rate and a detailed understanding of the mRNA transgene expression kinetics. Therefore, quantitative analysis of the time-dependent translation behavior would provide a better understanding of mRNA's transient nature and further aid the enhancement of appropriate carriers with the perspective to generate future precision nanomedicines with quick response to treat various diseases.

Results

A lipid-polymer hybrid system complexed with mRNA was evalu

SUBMITTER: Yasar H 

PROVIDER: S-EPMC6145106 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature