Impact of repeated anesthesia with ketamine and xylazine on the well-being of C57BL/6JRj mice.

ABSTRACT: Within the scope of the 3Rs of Russel and Burch, the number of laboratory animals can be reduced by repeated use of an animal. This strategy only becomes relevant, if the total amount of pain, distress or harm the individual animal experiences does not exceed the severity of a single manipulation. For example, when using imaging techniques, an animal can be examined several times during a study, but it has to be anesthetized each time imaging is performed. The severity of anesthesia is thought to be mild according to the Directive 2010/63/EU. However, the Directive does not differentiate between single and repeated anesthesia, although repeated anesthesia may have a greater impact on well-being. Hence, we compared the impact of single and repeated anesthesia (six times at an interval of th

SUBMITTER: Hohlbaum K 

PROVIDER: S-EPMC6145541 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature