Easy and efficient production of completely embryonic-stem-cell-derived mice using a micro-aggregation device.

ABSTRACT: There is an increasing demand for genetically modified mice produced without crossing, for rapid phenotypic screening studies at the organismal level. For this purpose, generation of completely embryonic-stem-cell (ESC)-derived chimeric mice without crossing is now possible using a microinjection or aggregation method with 3i culture medium. However, the microinjection of ESCs into blastocyst, morula, or 8-cell-stage embryos requires a highly skilled operator. The aggregation method is an easier alternative, but the conventional aggregation protocol still requires special skills. To make the aggregation method easier and more precise, here we developed a micro-aggregation device. Unlike conventional 3-dimensional culture, which uses hanging-drop devices for aggregation, we fabricated a pol

SUBMITTER: Sumiyama K 

PROVIDER: S-EPMC6145547 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature