Ratio of mitochondrial to nuclear DNA affects contamination estimates in ancient DNA analysis.

ABSTRACT: In the last decade, ancient DNA research has grown rapidly and started to overcome several of its earlier limitations through Next-Generation-Sequencing (NGS). Among other advances, NGS allows direct estimation of sample contamination from modern DNA sources. First NGS-based approaches of estimating contamination measured heterozygosity. These measurements, however, could only be performed on haploid genomic regions, i.e. the mitochondrial genome or male X chromosomes, but provided no measures of contamination in the nuclear genome of females with their two X chromosomes. Instead, female nuclear contamination is routinely extrapolated from mitochondrial contamination estimates, but it remains unclear if this extrapolation is reliable and to what degree variation in mitochondrial to nuclear

SUBMITTER: Furtwangler A 

PROVIDER: S-EPMC6145933 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature