Hyaluronic acid hydrogel scaffolds loaded with cationic niosomes for efficient non-viral gene delivery.

ABSTRACT: The lack of ideal non-viral gene carriers has motivated the combination of delivery systems and tissue-engineered scaffolds, which may offer relevant advantages such as enhanced stability and reduced toxicity. In this work, we evaluated a new combination between niosome non-viral vectors and hyaluronic acid (HA) hydrogel scaffolds, both widely studied due to their biocompatibility as well as their ability to incorporate a wide variety of molecules. We evaluated three different niosome formulations (niosomes 1, 2 and 3) varying in composition of cationic lipid, helper lipid and non-ionic tensioactives. Niosomes and nioplexes obtained upon the addition of plasmid DNA were characterized in terms of size, polydispersity, zeta potential and ability to transfect mouse bone m

SUBMITTER: Villate-Beitia I 

PROVIDER: S-EPMC6146377 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature