Functional Genetic Elements for Controlling Gene Expression in Cupriavidus necator H16.

ABSTRACT: A robust and predictable control of gene expression plays an important role in synthetic biology and biotechnology applications. Development and quantitative evaluation of functional genetic elements, such as constitutive and inducible promoters as well as ribosome binding sites (RBSs), are required. In this study, we designed, built, and tested promoters and RBSs for controlling gene expression in the model lithoautotroph Cupriavidus necator H16. A series of variable-strength, insulated, constitutive promoters exhibiting predictable activity within a >700-fold dynamic range was compared to the native P _phaC , with the majority of promoters displaying up to a 9-fold higher activity. Positively (AraC/P _araBAD -l-arabinose and RhaRS/P _rhaBAD

SUBMITTER: Alagesan S 

PROVIDER: S-EPMC6146998 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature