Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus.

ABSTRACT: Rod-shaped bacteria typically grow first via sporadic and dispersed elongation along their lateral walls and then via a combination of zonal elongation and constriction at the division site to form the poles of daughter cells. Although constriction comprises up to half of the cell cycle, its impact on cell size control and homeostasis has rarely been considered. To reveal the roles of cell elongation and constriction in bacterial size regulation during cell division, we captured the shape dynamics of Caulobacter crescentus with time-lapse structured illumination microscopy and used molecular markers as cell-cycle landmarks. We perturbed the constriction rate using a hyperconstriction mutant or fosfomycin ([(2R,3S)-3-methyloxiran-2-yl]phosphonic acid) inhibition. We report that the constric

SUBMITTER: Lambert A 

PROVIDER: S-EPMC6147026 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature