Ras Post-transcriptionally Enhances a Pre-malignantly Primed EMT to Promote Invasion.

ABSTRACT: Epithelial-to-mesenchymal transition (EMT) is integral to cancer progression, with considerable evidence that EMT has multiple intermediary stages. Understanding the mechanisms of this stepwise activation is of great interest. We recreated a genetically defined model in which primary cells were immortalized, resulting in migratory capacity, and subsequently H-Ras-transformed, causing malignancy and invasion. To determine the mechanisms coordinating stepwise malignancy, we quantified the changes in messenger RNA (mRNA) and protein abundance. During immortalization, we found dramatic changes in mRNA, consistent with EMT, which correlated with protein abundance. Many of these same proteins also changed following Ras transformation, suggesting that pre-malignant cells were primed for malignant

SUBMITTER: Bisogno LS 

PROVIDER: S-EPMC6147080 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature