Comprehensive molecular characterization of microneedling therapy in a human three-dimensional skin model.

ABSTRACT:

Background and objectives

Microneedling therapy is a widely used technique in dermatology. However, little is known about the underlying molecular effects of this therapy on extracellular matrix remodeling, wound healing, and inflammation. The aim of this study was to examine morphological and molecular changes caused by microneedling treatment in a standardized in vitro full-thickness 3D model of human skin.

Materials and methods

A microneedling device was used to treat full-thickness 3D skin models. Specimens were harvested at specified time points and qRT-PCR and microarray studies were performed. Frozen sections were examined histologically.

Results

Microneedling treatment caused morphological changes in the skin model resulting in an almost complete recovery of t

SUBMITTER: Schmitt L 

PROVIDER: S-EPMC6147558 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature