Identification of endogenous normalizing genes for expression studies in inguinal ring tissue for scrotal hernias in pigs.

ABSTRACT: The use of reference genes is required for relative quantification in gene expression analysis and since the stability of these genes could be variable depending on the experimental design, it has become indispensable to test the reliability of endogenous genes. Therefore, this study evaluated 10 reference candidate genes in two different experimental conditions in order to obtain stable genes to be used as reference in expression studies related to scrotal hernias in pigs. Two independent experiments were performed: one with 30 days-old MS115 pigs and the other with 60 days-old Landrace pigs. The inguinal ring/canal was collected, frozen and further submitted to real-time PCR analysis (qPCR). For the reference genes stability evaluation, four tools were used: GeNorm in the SLqPCR, BestKee

SUBMITTER: Lorenzetti WR 

PROVIDER: S-EPMC6147718 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature