Performance analysis of automated evaluation of antinuclear antibody indirect immunofluorescent tests in a routine setting.

ABSTRACT:

Purpose

Indirect immunofluorescence (IIF) on the human epithelial cell-line HEp-2 (or derivatives) serves as the gold standard in antinuclear antibody (ANA) screening. IIF, and its evaluation, is a labor-intensive method, making ANA testing a major challenge for present clinical laboratories. Nowadays, several automated ANA pattern recognition systems are on the market. In the current study, the EUROPattern Suite is evaluated for its use in daily practice in a routine setting.

Methods

A total of 1033 consecutive routine samples was used to screen for ANA. Results (positive/negative ANA screening, pattern identification and titer) were compared between software-generated results (EUROPattern) and visual interpretation (observer) of automatically acquired digital images.

Re

SUBMITTER: van Beers JJBC 

PROVIDER: S-EPMC6147779 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature