Ultrafast Volumetric Optoacoustic Imaging of Whole Isolated Beating Mouse Heart.

ABSTRACT: The Langendorff-perfused heart technique has become the model of choice for multiparametric optical mapping of cardiac function and electrophysiology. However, photon scattering in tissues represents a significant drawback of the optical imaging approach, fundamentally limiting its mapping capacity to the heart surface. This work presents the first implementation of the optoacoustic approach for 4D imaging of the entire beating isolated mouse heart. The method combines optical excitation and acoustic detection to simultaneously render rich optical contrast and high spatio-temporal resolution at centimeter-scale depths. We demonstrate volumetric imaging of deeply located cardiac features, including the interventricular septum, chordae tendineae, and papillary muscles while further tracking

SUBMITTER: Lin HA 

PROVIDER: S-EPMC6148063 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature