Kinetics of the invasion and egress processes of Babesia divergens, observed by time-lapse video microscopy.

ABSTRACT: Based on confocal fluorescence and bright field video microscopy, we present detailed observations on the processes of invasion and egress of erythrocytes by the apicomplexan parasite Babesia divergens. Time-lapse images reveal numerous unexpected findings associated with the dynamics of B. divergens and its ability to manipulate the erythrocyte during both processes in its asexual cycle under in vitro conditions. Despite the speed at which these processes occur and the small size of the parasite, we capture infective merozoites moving vigorously and causing striking deformations in the erythrocyte's plasma membrane during an active invasion. We also observed intraerythrocytic dynamic stages as paired pyriforms, double paired pyriforms, tetrads, unattached pyriform sister cells and multipl

SUBMITTER: Sevilla E 

PROVIDER: S-EPMC6148197 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature