Unknown

Dataset Information

0

NADPH oxidase 4 regulates anoikis resistance of gastric cancer cells through the generation of reactive oxygen species and the induction of EGFR.

ABSTRACT: Anoikis is a type of programmed cell death induced by detachment from the extracellular matrix. In cancer cells, anoikis resistance is essential for cancer cell survival in blood circulation and distant metastasis. However, the mechanisms behind anoikis resistance of gastric cancer remain largely unknown. Herein, we demonstrate that NADPH oxidase 4 (NOX4) expression and reactive oxygen species (ROS) generation are upregulated in suspension gastric cell cultures compared with adherent cultures. Silencing of NOX4 decreases ROS generation and downregulates EGFR, sensitizing cells to anoikis. NOX4 overexpression upregulates ROS and EGFR levels and promotes anoikis resistance. NOX4 depletion inhibits gastric cancer survival in blood circulation and attenuates distant metastasis. NOX4 expression is correlated with EGFR expression in patients. In conclusion, induction of NOX4 expression by detachment promotes anoikis resistance of gastric cancer through ROS generation and downstream upregulation of EGFR, which is critical for the metastatic progression of gastric cancer.

SUBMITTER: Du S 

PROVIDER: S-EPMC6148243 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

NADPH oxidase 4 regulates anoikis resistance of gastric cancer cells through the generation of reactive oxygen species and the induction of EGFR.

Du Shangce S   Miao Ji J   Zhu Zhouting Z   Xu En E   Shi Linsen L   Ai Shichao S   Wang Feng F   Kang Xing X   Chen Hong H   Lu Xiaofeng X   Guan Wenxian W   Xia Xuefeng X  

Cell death & disease 20180920 10

Anoikis is a type of programmed cell death induced by detachment from the extracellular matrix. In cancer cells, anoikis resistance is essential for cancer cell survival in blood circulation and distant metastasis. However, the mechanisms behind anoikis resistance of gastric cancer remain largely unknown. Herein, we demonstrate that NADPH oxidase 4 (NOX4) expression and reactive oxygen species (ROS) generation are upregulated in suspension gastric cell cultures compared with adherent cultures. S  ...[more]

Similar Datasets

Unknown Endothelin signalling regulates volume-sensitive Cl- current via NADPH oxidase and mitochondrial reactive oxygen species.
| S-EPMC2936123 | biostudies-literature
Unknown NADPH oxidase activation is required in reactive oxygen species generation and cell transformation induced by hexavalent chromium.
| S-EPMC3179675 | biostudies-literature
Unknown Urotensin-II-Mediated Reactive Oxygen Species Generation via NADPH Oxidase Pathway Contributes to Hepatic Oval Cell Proliferation.
| S-EPMC4676694 | biostudies-literature
Unknown Emodin-induced generation of reactive oxygen species inhibits RhoA activation to sensitize gastric carcinoma cells to anoikis.
| S-EPMC2213898 | biostudies-literature
Unknown Endothelium-dependent coronary vasodilatation requires NADPH oxidase-derived reactive oxygen species.
| S-EPMC2924465 | biostudies-literature
Unknown Primary involvement of NADPH oxidase 4 in hypoxia-induced generation of reactive oxygen species in adipose-derived stem cells.
| S-EPMC3411360 | biostudies-literature
Unknown Yno1p/Aim14p, a NADPH-oxidase ortholog, controls extramitochondrial reactive oxygen species generation, apoptosis, and actin cable formation in yeast.
| S-EPMC3365156 | biostudies-literature
Unknown SUMO1 negatively regulates reactive oxygen species production from NADPH oxidases.
| S-EPMC3464053 | biostudies-literature
Unknown Essential role of NADPH oxidase-dependent reactive oxygen species generation in regulating microRNA-21 expression and function in prostate cancer.
| S-EPMC3852344 | biostudies-literature
Unknown Caveolin-1 is a negative regulator of NADPH oxidase-derived reactive oxygen species.
| S-EPMC4228786 | biostudies-literature