Coherent Feedforward Regulation of Gene Expression by Caulobacter σ^T and GsrN during Hyperosmotic Stress.

ABSTRACT: GsrN is a conserved small RNA that is under transcriptional control of the general stress sigma factor, σ^T, and that functions as a posttranscriptional regulator of Caulobacter crescentus survival under multiple stress conditions. We have defined features of GsrN structure that determine survival under hyperosmotic stress, and we have applied transcriptomic and proteomic methods to identify regulatory targets of GsrN under hyperosmotic conditions. The 5' end of GsrN, which includes a conserved cytosine-rich stem-loop structure, is necessary for cell survival after osmotic upshock. GsrN both activates and represses gene expression in this stress condition. Expression of an uncharacterized open reading frame predicted to encode a glycine zipper protein, osrP, is stro

SUBMITTER: Tien MZ 

PROVIDER: S-EPMC6148470 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature