Fluorescence-Based Detection of Natural Transformation in Drug-Resistant Acinetobacter baumannii.

ABSTRACT: Acinetobacter baumannii is a nosocomial agent with a high propensity for developing resistance to antibiotics. This ability relies on horizontal gene transfer mechanisms occurring in the Acinetobacter genus, including natural transformation. To study natural transformation in bacteria, the most prevalent method uses selection for the acquisition of an antibiotic resistance marker in a target chromosomal locus by the recipient cell. Most clinical isolates of A. baumannii are resistant to multiple antibiotics, limiting the use of such selection-based methods. Here, we report the development of a phenotypic and selection-free method based on flow cytometry to detect transformation events in multidrug-resistant (MDR) clinical A. baumannii isolates. To this end, we e

SUBMITTER: Godeux AS 

PROVIDER: S-EPMC6148472 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature