Redirection of Metabolism in Response to Fatty Acid Kinase in Staphylococcus aureus.

ABSTRACT: Staphylococcus aureus is capable of phosphorylating exogenous fatty acids for incorporation into the bacterium's membrane via the fatty acid kinase, FakA. Additionally, FakA plays a significant role in virulence factor regulation and skin infections. We previously showed that a fakA mutant displays altered growth kinetics in vitro, observed during the late-exponential phase of growth. Here, we demonstrate that the absence of FakA leads to key metabolic changes. First, the fakA mutant has an altered acetate metabolism, with acetate being consumed at an increased rate than in the wild-type strain. Moreover, the growth benefit was diminished with inactivation of the acetate-generating enzyme AckA. Using a mass spectrometry-based approach, we identified altered conc

SUBMITTER: DeMars Z 

PROVIDER: S-EPMC6148474 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature