Custom made inclusion bodies: impact of classical process parameters and physiological parameters on inclusion body quality attributes.

ABSTRACT:

Background

The bacterium E. coli is a major host for recombinant protein production of non-glycosylated products. Depending on the expression strategy, the recombinant protein can be located intracellularly. In many cases the formation of inclusion bodies (IBs), protein aggregates inside of the cytoplasm of the cell, is favored in order to achieve high productivities and to cope with toxic products. However, subsequent downstream processing, including homogenization of the cells, centrifugation or solubilization of the IBs, is prone to variable process performance or can be characterized by low extraction yields as published elsewhere. It is hypothesized that variations in IB quality attributes (QA) are responsible for those effects and that such attributes can be controlled by ups

SUBMITTER: Slouka C 

PROVIDER: S-EPMC6148765 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature