Single-cell transcriptomics reveal that PD-1 mediates immune tolerance by regulating proliferation of regulatory T cells.

ABSTRACT:

Background

We have previously reported an antigen-specific protocol to induce transplant tolerance and linked suppression to human embryonic stem cell (hESC)-derived tissues in immunocompetent mice through coreceptor and costimulation blockade. However, the exact mechanisms of acquired immune tolerance in this model have remained unclear.

Methods

We utilize the NOD.Foxp3^hCD2 reporter mouse line and an ablative anti-hCD2 antibody to ask if CD4⁺FOXP3⁺ regulatory T cells (Treg) are required for coreceptor and costimulation blockade-induced immune tolerance. We also perform genome-wide single-cell RNA-sequencing to interrogate Treg during immune rejection and tolerance and to indicate possible mechanisms involved in sustaining Treg function.

R

SUBMITTER: Leung CS 

PROVIDER: S-EPMC6148788 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature