A fluorescent methylation-switchable probe for highly sensitive analysis of FTO N ⁶-methyladenosine demethylase activity in cells.

ABSTRACT: N ⁶-Methyladenosine (m⁶A) is one of the most abundant epigenetic modifications on mRNA. It is dynamically regulated by the m⁶A demethylases FTO and ALKBH5, which are currently attracting intense medical interest because of their strong association with several human diseases. Despite their clinical significance, the molecular mechanisms of m⁶A demethylases remain unclear, hence there is tremendous interest in developing analytical tools to facilitate their functional studies, with a longer term view of validating their therapeutic potentials. To date, no method exists which permits the analysis of m⁶A-demethylase activity in cells. To overcome this challenge, herein, we describe the first example of a fluorescent m⁶A-

SUBMITTER: Cheong A 

PROVIDER: S-EPMC6149071 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature